منابع مشابه
Resolution of the cytochrome P-450-containing omega-hydroxylation system of liver microsomes into three components.
The enzyme system in liver microsomes which catalyzes the w-hydroxylation of fatty acids in the presence of molecular oxygen and a reduced pyridine nucleotide has been solubilized and resolved into fractions containing cytochrome P-450, a cytochrome P-450 reductase, and a heat-stable factor. The apparent K, values of TPNH and DPNH are 2.0 x 10m5 M and 6.3 X 10e4 M, respectively. As a further in...
متن کاملCytochrome P-450 Reductase from Adrenocortical Microsomes
The interaction between P-450czl and NADPH-cytochrome P-450 reductase, both purified from bovine adrenocortical microsomes, has been investigated in a reconstituted system with a nonionic detergent, Emulgen 913, by kinetic analysis and gel filtrations. Steady state kinetic data in progesterone 2 1-hydroxylation showed formation of an equimolar complex between the two enzyme proteins at low Emul...
متن کاملA new cytochrome in liver microsomes.
8. HEPPEL, L. A., in S. P. COLOWICK AND N. 0. KAPLAN (Editors), Methods in enzymology, Vol. II, Academic Press, Inc., New York, 1955, p. 575. CANTONI, G. L., in M. FLORKIN AND H. S. MASON (Editors), Comparative biochemistry, Vol. I, Academic Press, Inc., New York, 1960, p. 181. THEORELL, H., in D. E. GREEN, (Editor), Currents in biochemical Research, Interscience Publishers, Inc., New York, 195...
متن کاملElectron Transport Components of Hepatic Microsomes
Components of the hepatic microsomal P-450 hemoprotein electron transfer system (TPNH-cytochrome c reductase and P-450 hemoprotein) and the microsomal cytochrome b5 electron transfer system (DPNH-cytochrome bs reductase and cytochrome bS) were solubilized using Triton N-101 and glycerol, and separated using DEAE-cellulose chromatography. Microsomal aniline hydroxylase activity was reconstituted...
متن کاملInhibitory effects of seven components of danshen extract on catalytic activity of cytochrome P450 enzyme in human liver microsomes.
The potential for herb-drug interactions has recently received greater attention worldwide, considering the fact that the use of herbal products becomes more and more widespread. The goal of this work was to examine the potential for the metabolism-based drug interaction arising from seven active components (danshensu, protocatechuic aldehyde, protocatechuic acid, salvianolic acid B, tanshinone...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1975
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1975.tb02167.x